Fig. 4: A1AR activation contributes to the altered transcriptional profile of astrocytes upon peripheral LPS challenge. | Nature Communications

Fig. 4: A1AR activation contributes to the altered transcriptional profile of astrocytes upon peripheral LPS challenge.

From: Adenosine triggers early astrocyte reactivity that provokes microglial responses and drives the pathogenesis of sepsis-associated encephalopathy in mice

Fig. 4

a Heatmap of altered gene expression (Padj <0.05) from astrocytic mRNARiboTag of male Adora1 cKO and ctl mice in any of the three time points (n = 3 mice per group). Clustering was done with 8 K-means. b Selected GSEA-KEGG pathway analysis of the astrocytic RNA-seq dataset between the Adora1 cKO and ctl groups at 6 hpi. c Selected GSEA plot of the enriched KEGG pathways related to inflammation between the Adora1 cKO and ctl groups at 6 hpi. d Selected GSEA-GO pathway analysis of the astrocytic RNA-seq dataset between the Adora1 cKO and ctl groups at 6 hpi. e Category net plot of selected enriched GO pathways relative to G protein-coupled receptor signaling pathway. The color gradient indicates the fold changes between the Adora1 cKO and ctl groups. f Prediction of transcription regulators following expression pattern of sub-clusters (cluster 1 and 5) by Metascape analysis77,78. Statistical significance in a was assessed by wald test using Benjamini and Hochberg method, in b, d, f was assessed by hypergeometric test with Benjamini and Hochberg method. Source data are provided as a Source Data file.

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