Fig. 2: Burs and PBurs are required for metabolic and developmental adaptation to a high-sugar diet in Drosophila. | Nature Communications

Fig. 2: Burs and PBurs are required for metabolic and developmental adaptation to a high-sugar diet in Drosophila.

From: LGR signaling mediates muscle-adipose tissue crosstalk and protects against diet-induced insulin resistance

Fig. 2

a, b Pupariation timing on normal diet (ND) and high-sugar diet (HSD) for controls and BursZ4410 heterozygotes and homozygous mutants (a), and for animals with loss of Burs in Burs-expressing cells (b). c–e Stored lipid levels in animals raised for 90 h on ND and then fed a 10-hour pulse of ND or HSD, comparing controls and animals with Burs knockdown in Burs-expressing cells (c) or pan-neuronal Burs (d) or Pburs (e) loss. Experiments measuring TAG levels in animals lacking Burs and Pburs were performed concurrently and share the control but are presented in separate panels (Fig. 2d, e) for clarity. f Whole-body 4EBP transcript levels measured by qPCR in controls and animals with Burs loss in the Burs-expressing cells, raised on ND for 90 h and then fed with a 10-hour pulse of ND or HSD. g Whole-body pAkt levels, normalized to Tubulin, measured by Western blot, in controls and Burs-cell Burs-deletion animals fed ND or HSD for ten h after 90 h on ND. h, i Hemolymph glucose levels of control and Burs-cell Burs-loss animals raised on HSD (h), and raised on ND then exposed to a 10-hour pulse of HSD (i). Animals in (a) were raised at 25 °C, while all others were raised at 29 °C to enhance RNAi and CRISPR efficiencies. Data are presented as means; SEM is shown with shaded areas in (a, b) and error bars in other figures. All data are from biologically independent replicates, with sample sizes indicated on graphs. ns, not significant (p > 0.05). a one-way Kruskal-Wallis nonparametric ANOVA with Dunn’s multiple-comparison test between 50%-pupariation times and two-way ANOVA for interaction. 50% pupariation time was determined via linear interpolation between adjacent observations below/before and above/after 50%. b, one-way ANOVA with Tukey multiple comparisons between 50%-pupariation times and two-way ANOVA for interaction. c, two-sided Mann-Whitney nonparametric U test. d, e, f, h, i: one-way ANOVAs with Dunnett’s multiple-comparison test. g, two-sided unpaired parametric t-test. Source data are provided as a Source Data file.

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