Fig. 4: CD244bright CD8bright NK cell subset is significantly expanded in peripheral blood of birdshot uveitis patients. | Nature Communications

Fig. 4: CD244bright CD8bright NK cell subset is significantly expanded in peripheral blood of birdshot uveitis patients.

From: Single-cell profiling identifies a CD8bright CD244bright Natural Killer cell subset that reflects disease activity in HLA-A29-positive birdshot chorioretinopathy

Fig. 4

A t-SNE plot of flow cytometry data from Healthy control (HC) n = 11, birdshot uveitis (BCR-UV) n = 18. Clusters identified by FlowSOM analysis (n = 12 clusters) are shown. B Comparison of the frequency of NK clusters between BCR-UV and Healthy controls. The -log10 (nominal P values) are from a two-sided likelihood ratio test. Significantly different clusters 0, 4, and 5 are highlighted. Source data are provided as a Source Data file. C Scatter plot of the frequency of cells of cluster 0 in peripheral blood in patients versus controls. HC n = 11, BCR-UV n = 18. ***P = 0.002. Data are presented as mean values ± SEM. Source data are provided as a Source Data file. D Heatmap showing the mean fluorescent intensity (MFI) of surface markers in NK clusters. Source data are provided as a Source Data file. E Principal component analysis (PCA) of NK clusters based on expression of surface markers. Source data are provided as a Source Data file. F Representative dot plots and corresponding frequency of CD8a+CD244+ NK cells in the peripheral blood of Healthy controls (HC, blue) and birdshot uveitis patients (BCR-UV, red). HC, n = 10; BCR-UV, n = 15. Statistical comparison is done using two-sided unpaired t test. ***P = 0.0003. Data are presented as mean values ± SEM. Source data are provided as a Source Data file.

Back to article page