Fig. 2: HLA-DR4^{Vim-64cit59-71}- and HLA-DR4^{α-eno-15cit10-22}-specific CD4⁺ T cells in RA donor.

a HLA-DR4^{Vim-64cit59-71} and HLA-DR4^{α-eno-15cit10-22} tetramer staining on CD4⁺ T cells post tetramer-based magnetic enrichment of PBMC from a HLA-DR4⁺ ACPA⁺ RA donor. Dot plots show CD14^-CD19^-live CD3⁺ CD4⁺ cells. Gating strategy is shown in Supplementary Fig. 1b. Arrows indicate cells designated RA2 A03 and RA2.7. b 293 T cells transiently co-transfected with RA2.7 TCR and CD3γδεζ or SKW-3 T cells transiently co-transfected with RA2 A03 TCR and CD3γδεζ were stained with either HLA-DR4^{α-eno-15cit10-22} tetramer (orange), HLA-DR4^{Vim-64cit59-71} tetramer (red) or control HLA-DR4^{Fibβ-74cit69-81} tetramer (charcoal). c TCR gene segment usage and CDR3 amino acid sequence for RA2 A03 and RA2.7 clones. CDR3 nucleotide sequences are listed in Supplementary Table 10.