Fig. 1: Nanog^BiD facilitates somatic cell reprogramming.

a Architecture of the synthetic factors. The N-terminus 70aa domain from SS18 was fused to the N-terminus of full-length transcription factors. b Schematic diagram illustrating the process of synthetic BiD factors induced cell reprogramming. c Heatmap of numbers for Oct4-GFP⁺ colonies of MEF cells reprogramming by different combinations. Red rectangles represent mean data for n = 3 biological replicates. d Number of iPSC colonies induced from MEFs infected by Nanog^BiD+Oct4 under different medium switch strategies. Bars are mean with SD, and red plots are individual data points for n = 4 biological replicates. Statistical significance relevant to number of Oct4-GFP⁺ colonies at Day 7 was measured with two-tailed unpaired t-test. P values were performed on graph. NS, nonsignificant. e Number of Oct4-GFP⁺ colonies during the process of Oct4+Sox2+Klf4, Nanog^BiD+Oct4, Nanog+Oct4^BiD, Nanog^BiD+Oct4^BiD, and Nanog+Oct4 mediated reprogramming. Bars are mean ± SD for n = 3 biological replicates. Statistical significance relative to colonies number of Nanog+Oct4 at Day 12 was measured with two-tailed unpaired t-test. P values were performed on graph. f Representative images of different reprogramming strategies induced colonies on day 12. Scale bar, 5 mm. g Detection of plasmid integration by PCR. Gels shown are representative of n = 2 independent experiments. Source data are provided as a Source Data file.