Fig. 2: Nanog^BiD interacts with the BAF complex to enhance reprogramming efficiency.

a Proteins of reprogramming samples at day 1 performed in two replicates for FLAG pull-down followed by MS analysis. b Venn diagram shows numbers of protein common and specific identified in Nanog^WT or Nanog^BiD associated reprogramming samples. Proteins are listed as official gene symbols. c Flag Co-IP followed by western blot analysis of SMARCC1, SMARCA4, FLAG, and NANOG. Blots shown are representative of n = 2 independent experiments. d Percentage of homogeneous Oct4-GFP positive colonies induced in Nanog^BiD, Nanog^BiD(3M), and Nanog^WT mediated reprogramming at different time points. Data are presented as mean ± SD for n = 3 biological replicates. Statistical significance relative to colonies number of Nanog^WT at Day 12 was measured with two-tailed unpaired t-test and precise p values are shown on graph. e Representative images of different reprogramming strategies induced colonies on day 12. Scale bar, 5 mm. Source data are provided as a Source Data file.