Fig. 5: Mutagenesis analysis of ASB7-mediated degradation in cells.
From: Molecular insights into degron recognition by CRL5ASB7 ubiquitin ligase
a–f Stability analysis of the GFP-fused LZTS1-degron was performed in ASB7 knockout HEK293T cells with overexpression of exogenous gASB7-resistant wild-type and mutant ASB7. The GFP/DsRed ratio was analyzed by flow cytometry. g Western blot analysis of Flag-tagged WT and mutant ASB7 expression in GPS reporter cell lines. Source data are provided as a Source Data file. Representative image, n = 3. h–m Stability analysis of GFP-fused CEP152-degron with overexpression of exogenous gASB7-resistant wild-type and mutant ASB7 in ASB7 knockout HEK293T cells. n Stability comparison of GFP-fused LZTS1-degron with substitutions in contact site 1, monitored by global protein stability assay. Source data are provided as Source Data file.
