Fig. 2: FAM92A1 depletion leads to abnormal brain structure and degeneration of hippocampal neurons. | Nature Communications

Fig. 2: FAM92A1 depletion leads to abnormal brain structure and degeneration of hippocampal neurons.

From: Membrane remodeling by FAM92A1 during brain development regulates neuronal morphology, synaptic function, and cognition

Fig. 2

a, b Representative MRIs of brains (a) and quantification of total ventricular areas (b) for each FAM92A1 genotype. n = 12 mice per group; one-way ANOVA. c Visualization of the anatomical localization of significant changed rGMV among wild-type and FAM92A1-deficient groups. The red and orange colors represent two significant changed areas. Color bar represents the F-value of one-way ANOVA; n = 8 mice per group. ENTm, medial entorhinal area; RSPd, dorsal retrosplenial area; RSPv, ventral retrosplenial area; cc, corpus callosum; cing, cingulum bundle. d Visualization of GMD in horizontal, sagittal, and coronal planes, respectively. Color bar represents GMD. e Scatter plots showing the GMD of each group. Data present as mean ± SEM; n = 8 mice per group; one-way ANOVA. f Representative images from four mice per group showing CV staining of five selected coronal planes (a–e) along the rostro-caudal axis of adult mouse brains, according to the Franklin and Paxinos’s Mouse Brain atlas. a anterior section without the corpus callosum; (b) anterior section including the corpus callosum; (c) medial section with a visible anterior commissure; (d) medial section including the just appeared hippocampus; (e) medial section including the clear DG area. Scale bar, 1000 μm. g Magnified CV staining of hippocampus and its subregions (CA1, CA3, and DG) from (f). Scale bar, 200 μm. h Box-and-whisker plot showing the thickness of the DG. n = 28 measurements from four mice per group; one-way ANOVA. For (b, h) data are presented as minimum to maximum values (whiskers) and the 25th to 75th percentiles (box), with the mean value within the box. i Heatmap showing the average change ratio of GMD within hippocampal subregions relative to the FAM92A1+/+ group. n = 8 mice per group, two-way ANOVA. j Representative images from three mice per group showing FJB staining of the hippocampus. Scale bar, 100 μm (top two panels) and 10 μm (magnification). *p < 0.05, **p < 0.01, and ***p < 0.001. Source data and exact p-values are provided as a Source Data file.

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