Fig. 3: FAM92A1 deficient mice present age-associated memory decline and cognitive deficits. | Nature Communications

Fig. 3: FAM92A1 deficient mice present age-associated memory decline and cognitive deficits.

From: Membrane remodeling by FAM92A1 during brain development regulates neuronal morphology, synaptic function, and cognition

Fig. 3

a Experimental procedure for behavioral tests with neuron-specific FAM92A1 knockdown mice. b Representative images showing EGFP expression in mouse HPC. Scale bar, 1 mm. c, d Western blot (c) and quantification (d) of FAM92A1 expression in mouse HPC. Data represent mean ± SEM; n = 5 mice per group; one-way ANOVA. e Experimental diagram for the MWM test. f, g Latency to find the platform during 5 days of training for FAM92A1 knockout (f) and knockdown mice (g). h, i Percentage of time spent in the four quadrants during the probe trial by FAM92A1 knockout mice (h) and knockdown mice (i). For (f–i) data represent mean ± SEM; n = 8 mice per group; two-way ANOVA. j, k Representative heatmaps and swimming trajectories of FAM92A1 knockout (j) and knockdown mice (k) during the probe trial. l Experimental diagram for fear conditioning test. m, n Percentage of freezing time in FAM92A1 knockout mice (m, n = 8 mice per group) and knockdown mice (n, n = 7 mice per group) during contextual and cued memory tests. Data represent mean ± SEM; one-way ANOVA. o, p Representative images (o) and quantification (p) of FAM92A1 expression of two wild-type (n = 60 measurements) and APP/PS1 (n = 62 measurements) mice per group. Scale bar, 10 μm. q, r Representative western blot (q) and quantification (r) of FAM92A1 expression in the HPC of four young (2 month-old) and old (10 month-old) mice per group. For (p) and (r) data represent mean ± SEM; unpaired two-tailed Student’s t-test. s Volcano plot of DEGs (fold change > 1.5 and p < 0.05) from RNA-seq analysis upon loss of FAM92A1. t Bubble chart showing the enriched human diseases and disorders in IPA analysis using the DEGs in panel (s). Statistical tests for (s) and (t) were based on a two-sided t-test with P-value adjustment for multiple comparisons (Benjamini–Hochberg method) by DESeq2 (n = 3 biologically independent samples per group). *p < 0.05, **p < 0.01, and ***p < 0.001. Source data and exact p-values are provided as a Source Data file.

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