Fig. 1: DDA1 is an interaction partner of CSA.
From: The small CRL4CSA ubiquitin ligase component DDA1 regulates transcription-coupled repair dynamics
A, B Scatter plot of Log2 SILAC ratios of proteins isolated by GFP-pulldown in CSA-mC KI HCT116 cells. The experiments were conducted in duplicate with a label swap, comparing the GFP immunoprecipitation of mock-treated CSA-mC KI versus HCT116 cells (A) or UV-treated CSA-mC KI versus HCT116 cells (B). Proteins with Log2 SILAC ratio >0.6 (indicated by grey line) in both replicates were classified as specific CSA interactors. RNAPII subunits are indicated in green, PAF-1 subunits are indicated in light purple, proteins associated with RNAPII are indicated in brown, TFIIH subunits are indicated in grey, TRiC subunits are indicated in orange, the COP9 subunits in yellow, CRL subunits in dark purple and TC-NER factors are indicated in red. C IP of CSA-mClover and GFP-DDB2 using GFP beads in CSA-mC and GFP-DDB2 KI cells followed by immunoblotting for the indicated proteins. HCT116 cells were used as a control. The experiment was repeated two times with similar results. Source data are provided as a Source Data file.
