Fig. 6: MT3 expression is increased in the cingulate and decreased in the Caudate of HD brains.

A Immunofluorescent images of the caudate sections labeled for nuclei (DAPI-blue) and GFAP (green) to detect astrocytes (left), and MT (red-middle panel). A merged panel is shown on the right. Arrows indicate DAPI, GFAP and MT positive cells (MT-positive astrocytes) and arrowheads indicate MT negative astrocytes. The antibody detects MT2A and MT1 proteins. Scale bar = 50 μm. B Quantification of the percent of MT-positive astrocytes in the caudate. Unpaired one-tailed t-test with n = 6 for control and 7 for HD. Data are shown as mean ± SEM. P value = 0.3232. C Immunofluorescent images of the caudate labeled for nuclei (DAPI-blue) and GFAP (green) to detect astrocytes (left panel), and MT3 (red-middle panel). A merge of the three channels is shown on the right. Arrows indicate DAPI, GFAP and MT3 positive cells (MT3 positive astrocytes) and arrowheads indicate MT3 negative astrocytes. Scale bar = 50 μm. D Quantification of the percent of astrocytes that were MT3 positive in the caudate. Unpaired one-tailed t-test used with  n = 10 for control and 11 for HD. Data are shown as mean ± SEM. P value = <0.0001. E Same as D but for the cingulate. F Quantification of the percent of percent of astrocytes that were MT3 positive in the cingulate. Unpaired one-tailed t-test with n = 8 for control and 6 for HD. Data are shown as mean ± SEM. P value = 0.0405. For all IHC panels, control and HD images are shown on the top and bottom rows, respectively.