Fig. 2: Proteomic and transcriptomic analysis of ∆ettA vs. WT strains reveal post-transcriptional downregulation of aceB, aceA, fumC, yjbJ, and hchA genes expression.

a The WT, ∆ettA, and C_ettA strains were cultivated in MMAA medium containing 0.4 M NaCl. Cells were harvested, and proteins were extracted into two fractions: S₁₅ (proteins in the soluble extract) and P₁₅₀ (proteins in the pellet after centrifugation at 150,000 × g) along with total RNA. b Volcano plots depict proteomic analysis of the fractions S₁₅ and P₁₅₀ fractions. The ratios ∆ettA/WT (black) or C_ettA/WT (green) of protein quantity and their corresponding q-value (corrected p-value for false discovery rate using Benjamini–Hochberg test) are presented in log₂ and -log₁₀ scales, respectively. Proteins discussed in the text are named. Red dots indicate the proteins downregulated in the ∆ettA strain that were further studied. c Bar graph illustrating log₂ ratios of some deregulated genes in ∆ettA strain from transcriptomic analysis (∆ettA/WT in blue bars; C_ettA/WT in light blue bars). Brown-underlined genes are regulated by FlhDC while orange-underlined genes are regulated by FliA. d Bar graph displaying log₂ ratios of ∆ettA / WT in proteomic (burgundy red bars) and in transcriptomic (blue bars) of deregulated genes in ∆ettA strain that have been validated independently. e Motility tests of the three strains (WT, ΔettA, and C_ettA) on 0.3% agar plates containing MMAA medium, showing a reduced motility for the ΔettA strain. f Heatmaps of the YFP total fluorescence level of the same three strains with a yfp translational fusion inserted in the genome in frame with the aceB, aceA, fumC, yjbJ, and hchA genes (aceB:yfp, aceA:yfp, fumC:yfp, yjbJ:yfp and hchA:yfp) grown in MMAA medium or LB medium for fumC:yfp.