Fig. 1: Generalized synthetic route and whole-cell-based colorimetric TA screening for biobased furan-derived intermediate products, and specific activities of SMTA toward HMF and its derivatives.
From: Biocatalysis enables the scalable conversion of biobased furans into various furfurylamines
a Generalized synthetic route toward the synthesis of oxidized/reduced/aminated intermediate products from biobased furans. b Whole-cell-based colorimetric TA screening using 2-(4-Nitrophenyl)ethan-1-amine and HMF (R = -CH2OH), FFCA (R = -COOH), and DFF (R = -CHO) as an amino acceptor; screening conditions: described in the supplementary methods section. c Specific activities of SMTA toward HMF and HMF-based derivatives and substituted furfurals. Reaction conditions: 10 mM of amino acceptors, 100 mM of IPA, 0.1 mM of PLP, and 100 mM of Tris-HCl (pH 8.0), 37 °C. Source data is provided as a Source Data file. Data in c are mean values of triplicate experiments with error bars indicating the s. d. (n = 3).
