Fig. 1: Cajal-Retzius cells receive the bulk of their inputs from layer 1 GABAergic neurons. | Nature Communications

Fig. 1: Cajal-Retzius cells receive the bulk of their inputs from layer 1 GABAergic neurons.

From: Developmental Cajal-Retzius cell death contributes to the maturation of layer 1 cortical inhibition and somatosensory processing

Fig. 1

a Schematic figure for anatomical tracing, targeting CRs. bi-iv Examples of stained presynaptic partners (magenta in place of mCherry) of CRs (green) and starter cells (white) for Control and Bax cKO mice at P5-7 (Control N = 5, Bax cKO N = 4) and P21 (Control N = 2, Bax cKO N = 9), Scale bar = 100 μm. The layers have been standarized based on DAPI staining. The yellow boxes represent the zoomed in pictures of the starter cells (SC) on the left of each image. The boxes bellow the zoomed images show the percentage of presynaptic partners across layers. c, d One-way Anova was performed to compare the effect of CRs survival across development F(7,32) = 1.4, p = 0.0002 two-tailed. For each group all brain slices with starter cells and presynaptic partners were counted and averaged per brain (min 3 slices per brain). Data are presented as mean values +/- SD. e Schematic representation of the in vitro whole-cell patch-clamp recordings of CRs during electrical stimulation of L1. Increased stimulation (20-450 μV) was performed in L1 and the evoked inhibitory postsynaptic currents (eIPSCs) of the patched CRs were measured upon CNQX and APV application at Vm = 0 mV. f Average traces of inhibitory inputs received by CRs across groups and ages. g Multiple t-test (two-sided) analysis showed a significant difference between the Control CRs at P5-7 and the Bax cKO CRs at P21 across all stimulation paradigms. Using the same analysis a significant difference was observed for Bax cKO CRs at P5-7 vs P21 only for the initial stimulation paradigms (20-150μV). All groups at P5-7 have 6 cells/3 mice. At P21 the Control N = 2 cells/2 mice and the Bax cKO N = 6 cells/3 mice. Statistics could not be performed for the Control CRs at P21 since it was more challenging to obtain adequate numbers of mice for analysis (2 out of 6 patched mouse brains). Data are presented as mean values +/- SEM. The significance threshold was set to p < 0.05; different degrees of significance are indicated by asterisks (p < 0.05: *; p < 0.01: **p < 0.001: ***, ns stands for non-significant result). Source data are provided as a Source Data file.

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