Fig. 1: Cryo-EM structure of human Pdcd4 bound to the 40S subunit. | Nature Communications

Fig. 1: Cryo-EM structure of human Pdcd4 bound to the 40S subunit.

From: Human tumor suppressor protein Pdcd4 binds at the mRNA entry channel in the 40S small ribosomal subunit

Fig. 1

A Native gel assay showing a fluorescence-labeled-Pdcd4 binding to 40S subunit in the absence or presence of excess unlabeled Pdcd4 (residues 1-469) or its fragment (NTD:1-156, or CTD:157-469). B Fluorescence anisotropy competition assay showing dissociation of labeled Pdcd4-NTD from 40S upon titration of unlabeled Pdcd4 or Pdcd4-NTD. Error bars represent the mean ± SEM (n = 3). For the NTD, only two independent experiments were performed. The experiment was repeated at least three times with similar results. C, D Cryo-EM reconstruction of human Pdcd4 bound to the 40S small ribosomal subunit. E The structure of Pdcd4-NTD and its corresponding cryo-EM density map shown as mesh.

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