Fig. 4: Cryo-EM 3D reconstruction of a human Pdcd4-40S-eIF4A-eIF3-eIF1 complex.

A, B Overall view of the cryo-EM structure shown in different orientations to highlight Pdcd4. C, D Cryo-EM map to highlight the density at the mRNA entry site that we assigned to Pdcd4 and eIF4A. The sharped map is docked into a map low-pass filtered to 15 Å (transparent silver). E, F Models of Pdcd4-CTD and eIF4A (PDB:2ZU6, where one of eIF4As is omitted)4 fitted into the cryo-EM map to highlight the interaction of Pdcd4 with eIF4A, uS3, and eIF3g-RRM.