Fig. 1: LsAAE3 enzyme assays of α/β-L-ODAP formation.
From: Reply to: LsBOS utilizes oxalyl-CoA produced by LsAAE3 to synthesize β-ODAP in grass pea
Shown are measurements of the supernatant after removal of LsAAE3, removal of LsAAE3 followed by addition of LsBOS and the coupled reaction in the presence of both LsAAE3 and LsBOS. a concentrations of α- and β-ODAP produced. b Western blot showing LsAAE3 in the initial reaction (lane 1) and absent in the supernatant following filtration and addition of LsBOS (lanes 2–4), alongside Biorad Precision Plus Marker. c LC-MS profiles of assays showing no detectable oxalyl-CoA. The bottom trace shows oxalyl-CoA produced in vitro using purified LsAAE3 as described in Edwards et al.2. Source data are provided as a Source Data file.
