Fig. 4: Characterization of functional SNP rs2297550 by CRISPR-mediated editing in Daudi cells.
From: High-throughput identification of functional regulatory SNPs in systemic lupus erythematosus
A Using base-edited clones (4C/C, 3C/G, and 3G/G), mRNA levels of IKBKE and \(\beta\)-actin were measured by RT-qPCR, and B protein levels of IKK\(\epsilon\) (80 KDa) and GAPDH (37 KDa) were measured by Western blotting, performed once (mean \(\pm\) s.d, P values from one-way ANOVA corrected by Tukey’s multiple comparisons). Source data are provided as a Source Data file.
