Fig. 6: rs2297550 risk allele G bind Ikaros.
From: High-throughput identification of functional regulatory SNPs in systemic lupus erythematosus
A Oligonucleotide pulldown western blot assay testing Ikaros binding to rs2297550-G; binding is eliminated in the presence of a 30-fold excess of non-biotinylated rs2297550-C or G competitor; positive control, nuclear extract. Pulldown western blot was performed once. B EMSA supershift assay showed that anti-Ikaros caused fading of the shifted band (oligo rs2297550-G, Daudi nuclear extract), whereas isotype control did not; anti-E2F4 also exhibited specificity. EMSA supershift was repeated twice. All blots are provided in the Source Data. C ChIP-qPCR using anti-Ikaros confirms binding to rs2297550 (Daudi cells, mean \(\pm\) s.d, three biological replicates). D Allele discrimination ChIP-qPCR shows Ikaros preferentially binds to G allele over C allele of rs2297550 in CRISPR-HDR edited C/G Daudi cells (mean \(\pm\) s.d, three biological replicates). All studies were done in unstimulated cells. Statistical analysis for C and D: paired t-test with two tails, without correction. Source data are provided as a Source Data file.
