Fig. 5: Identification of key residues for DNA Binding in the ATP(+)-DNA(+)-MuB helical filament. | Nature Communications

Fig. 5: Identification of key residues for DNA Binding in the ATP(+)-DNA(+)-MuB helical filament.

From: Elucidating the Architectural dynamics of MuB filaments in bacteriophage Mu DNA transposition

Fig. 5

a Top view of one helical turn with dsDNA and interacting residues shown. b Interactions between DNA and MuB’s basic residues. c Side view of the helical turn with dsDNA and interacting residues shown. d Zoom in-view shows the interactions between DNA and the DNA-binding loop, with interaction distances (Å) shown. e DNA-binding assay by agarose-EMSA. WT, wild-type. Data points are shown as the mean ± SEM (n  =  3 independent experiments). Source data are provided as a Source Data file.

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