Fig. 3: Characterisation of the WD33 and WD34 interactions with AMA1.
a ELISA showing the binding of biotinylated RON2 peptide (150 nM) to immobilised AMA1 in the presence of i-body (150 nM). AMA1-bound RON2 peptide was measured using streptavidin HRP. Data are presented as mean values ± SD. b Inhibition of RON2 peptide (150 nM) binding to AMA1 by increasing concentrations of WD34. Data are presented as mean values ± SD. Source data are provided as a Source Data file. c Equilibrium dissociation constants (KD) of WD34 and WD33 interacting with AMA1 determined by surface plasmon resonance. All the generated sensorgrams were analysed by T200 evaluation software. At least three independent experiments were performed to get the final reported values. KD values are reported at the nanomolar level, and the standard deviations are reported in parenthesis. See the Supplementary SPR information. d Dose-dependent inhibition of WD34 binding to AMA1 by mAbs 1F9 and 4G2, which have been previously reported to bind around the hydrophobic cleft (e). Three independent experiments were performed. Data are presented as mean values ± SD.
