Fig. 5: WD34 inhibits both merozoite and sporozoite life-cycle stages. | Nature Communications

Fig. 5: WD34 inhibits both merozoite and sporozoite life-cycle stages.

From: A broadly cross-reactive i-body to AMA1 potently inhibits blood and liver stages of Plasmodium parasites

Fig. 5

a Inhibition of merozoite growth by WD34 was tested using multiple Plasmodium parasites. Assays were performed using two-fold dilutions of WD34 starting with 0.5 mg/ml. Data represents the mean of three independent experiments and error bars represent standard deviations. b Growth inhibition of multiple Plasmodium parasites by WD33. Assays were performed with two-fold dilutions of WD33 starting with 1 mg/ml. Three independent experiments were performed, and data points are represented as mean values ± SD. c IC50 values for each parasite line were calculated by using the non-linear regression model. d, e Effect of i-bodies on sporozoite traversal (d) invasion (e) of HC-04 hepatocytes by P. falciparum sporozoites. WD34 or 21H5 treated FITC-Dextran+HC-04 cells or HC-04 cells were incubated with sporozoites. Cell traversal and invasion were measured at 3 and 24 h, respectively. The mean of n = 3 biological triplicates and standard deviations (a, b) or standard errors of the mean (d, e) are shown. Statistical analysis: 2-way ANOVA with multiple comparisons. Source data are provided as a Source Data file.

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