Fig. 4: Genome-wide profile of donor integration and chromosomal translocation.

a Brief workflow of Tn5-based high-throughput genome-wide sequencing for integration of ssDNA donor or translocation at the DSB site. Genomic DNA was tagmented with Tn5, and genomic regions containing the inserted donor sequences and the translocated regions were amplified using the indicated primer pairs designed for integration and translocation, respectively. After nest-PCR, amplicons were barcoded and then sequenced. b Circos plots of genome-wide off-target integrations of donors and translocation junctions in edited cells. Off-target integrations and translocation junctions were binned to 0.1 Mb regions and plotted on a normalized scale (black bars). Green arrow, ssDNA donor on-target site. Blue arrow, bait primer targeting site. c The overall off-target integration and translocation rate were calculated as the percentage of off-target reads in HDR reads and the percentage of translocation reads in non-translocation (bait region) reads, respectively. Values reflect n = 2 (c) independent electroporation replicates. The sequences of all gRNAs, ssDNA donors and primers used are shown in Supplementary Data 1, 2 and 4. Source data are provided as a Source Data file.