Fig. 2: Aggrecan deposits regulate blood-borne molecule distribution between the ME and the ARH.
a–n Quantification of Acan (a, e, upper panels, 3-7 mice, means and s.e.m. indicated, two-tailed unpaired t test with Welch’s correction p = 0.065, t(2.052) = 3.65), **p = 0.0095, t(4.473) = 4.347) and area of ACAN (a, e (lower panels, 3-4 mice, ARH analyzed bilaterally, mean and s.e.m. are shown, two-tailed unpaired t test *p = 0.012, t(8) = 3.25; with Welch’s correction **p = 0.0049, t(6.158) = 4.277), i 3-8 mice, two-tailed unpaired t test, **p = 0.0015, t(20) = 3.67, k) and PNN (j 3-5 mice, two-tailed Mann–Whitney test, **p = 0.0048, l) deposits, and quantification of albumin ventrodorsal distribution in the ME/ARH region (b–d, f–h, l–n) in Acanhypo (a–d, 3 mice; left and right ARH/mice, mean and s.e.m. are shown, one-way ANOVA test, ME vs ARH *p = 0.0271; ACAN+ vs ARH *p = 0.0443, F(2,9) = 6.161), AcaniE5/+ (e–h 3 mice, left and right ARH/mice; means and s.e.m. are shown, one-way ANOVA test, ****p < 0.0001; ***p = 0.0006, F (2, 15) = 25.83) and ADAMTS-5ARH (i–n; left and right ARH analyzed, means and s.e.m. are shown; m, n 5 mice, one-way ANOVA test, **p = 0.0013, ACAN+ vs ARH p = 0.059, F (2, 15) = 9.950) mice and their control littermates. Dashed lines in b, f and l delineate the ventral boundary of the ARH. Scale bars: 50 μm. ME median eminence, ARH hypothalamic arcuate nucleus. Source data are provided as a Source Data file.
