Fig. 4: Interaction analysis between LMHO and albumin. | Nature Communications

Fig. 4: Interaction analysis between LMHO and albumin.

From: Nano-anticoagulant based on carrier-free low molecular weight heparin and octadecylamine with an albumin shuttling effect

Fig. 4

a Size distribution of LMHO interacting with albumin (1:1, 1:10, and 1:100). In vitro albumin binding assay using albumin-coated agarose gel columns (n = 3 independent samples, unpaired t-test for p value) (b) and native PAGE analysis (c) using fluorescence-labeled LMWH and LMHO molecules (arbitrary units, Arb. u). d Quantitation of fluorescence-labeled LMWH and LMHO bound to albumin protein (n = 3 independent samples, unpaired t-test for p value). e MD simulation to investigate the interaction between LMHO and albumin for 600 ps. Representative transmission electron microscopy (TEM) images (n = 3 independent experiments) (f) and bio-layer interferometry (BLI) results (g) between LMHO and albumin. Data are presented as mean values ± SD. Source data are provided as a Source Data file.

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