Fig. 3: MCA-IIs co-fractionate with the ER and decrease the accumulation of ubiquitinated proteins. | Nature Communications

Fig. 3: MCA-IIs co-fractionate with the ER and decrease the accumulation of ubiquitinated proteins.

From: Seed longevity is controlled by metacaspases

Fig. 3

a Immunoblots probed with α-MCA-II-a, α-BiP2, and α-BRI1 from protein extracts fractionated by sucrose gradient ultracentrifugation in the presence (lower blots) or absence (upper blots) of Mg2+. Red arrows indicate the corresponding bands. Note that MCA-II-a is known to get auto-activated by self-processing and these fragments could be detected (CP, cleavage product from autoactivated MCA-II-a; N = 3, n = 1 replicate, 7 DPG). b Representative immunoblot probed with α-UBIQUITIN11 (UBQ11) from seed protein extracts (50 seeds/genotype) antibody. The numbers indicate relative levels of ubiquitinated proteins compared to Ponceau S staining at the bottom (loading control). Left blot: WT (9 month-old seeds) and mca-II-KOc (seeds harvested at different time points; 3, 6, and 9 month-old). Right blot: WT, mca-II-df (the initial background used for CRISPR), and mca-II-KOc with 9 month-old seeds (N = 2, n = 1 replicates).

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