Fig. 5: MCA-IIs regulate the dynamics of lipid droplets. | Nature Communications

Fig. 5: MCA-IIs regulate the dynamics of lipid droplets.

From: Seed longevity is controlled by metacaspases

Fig. 5

Representative immunoblots probed with an α-OLE1 from seed proteins of WT, mca-II-KOc (KOc), and the Com line (MCA-II-apro:GFP-MCA-II-a; 3 month-old seeds). The numbers indicate relative levels compared to Ponceau S staining at the bottom (loading control; N = 3, n = 1 with 50 seeds/lane). b Representative confocal micrographs from WT, mca-II-KOc, and Com line counterstained with LipidTOX in the embryonic hypocotyl regions after a 2 day stratification. Insets (denoted 1 to 3) show details of lipid droplets; examples of lipid droplets are denoted with yellow arrowheads. Scale bars, 5 μm (1 μm for insets). Right, measurement of lipid droplet size. P-values were calculated by ordinary one-way ANOVA (N = 3, n ≥ 15 embryonic hypocotyls). RU, relative units. Source data are provided as a Source Data file.

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