Fig. 6: MCA–IIs regulate the levels of PUX10 in cells.

a Upper: immunoblot of protein extracts probed with α-GFP from seedlings expressing PUX10pro:PUX10-GFP in WT and mca-II-KOc background. Lower: PUX10-GFP decay in WT or mca-II-KOc following CHX treatment (time points after CHX addition are denoted in min; N = 3, n = 2 replicates with ≥ 10 seedlings per lane 5 DPG). FL, full-length PUX10; CP, cleavage product (minus N-terminus of PUX10). b Confocal images from root epidermal cells of PUX10pro:PUX10-GFP seedlings treated with 50 μM of CHX and MG132 for various times (2 DPG; time points after CHX addition are denoted in min). Scale bars, 50 μm (N = 3, n = 1 replicate with 3 seedlings each). c Immunoblot probed with α-GFP from seedlings expressing PUX10pro:PUX10-GFP in WT in the presence or absence of MG132, and representative confocal images of embryonic roots from seedlings expressing PUX10pro:PUX10-mCherry in WT (upper) and mca-II-KOc (lower). Scale bars, 7 μm. d Immunoblot of protein extracts with α-myc from embryonic roots expressing PUX10pro:PUX10-myc in WT and mca-II-KOc background complemented with MCA-II-a-GFP or the corresponding inactive variant (PD; N = 1, n = 1 replicate with ≥ 10 seedlings per lane 5 DPG).