Fig. 9: MCA–IIs regulate the ERAD pathway.
a Images of the indicated genotypes, which were mock-treated (DMSO), treated with a CDC48 inhibitor (CB-5083, 2 μM), and following a 2 day recovery (9 day-old seedlings) from CB-5083 treatment on DMSO-containing plates. Note the swelling root tip phenotype and the shorter root (after 2 d recovery) observed in mca-II-KOc (two lines), which is indicative of hypersensitivity to CB-5083 (insets). Scale bar, 20 mm (N = 3, n = 8–10 seedlings/genotype 7 DPG). b Representative confocal micrographs from WT or mca-II-KOc RPS5apro:Bri1-9-GFP embryonic roots. The plot at the bottom shows the quantification of the ratio of Bri1-9-GFP signal intensity on the plasma membrane compared to the cytoplasmic signal (“cyt”). P-value, two-tailed Mann Whitney test (N = 3, n = 8-10 seedlings/genotype 2 days after stratification). c Representative immunoblot probed with α-BRI1 from WT, bri1-9 and the septuple mca-II-KOc bri1-9 mutant (N = 2, n = 1 replicate with 1 seedling root per lane 2 days after stratification). d Representative phenotypes of WT, bri1-9, and the septuple mca-II-KOc bri1-9 mutant from a double-blind experiment where the genotype-phenotype link was established independently. Scale bar, 10 mm. Source data are provided as a Source Data file.
