Fig. 2: Effect of PAGln pre-incubation on ISO- and NE-induced β-arrestin2 recruitment to β2AR in HTLA cells.

A β-arrestin2 recruitment induced by increasing concentration of isoproterenol (ISO) alone (Blue) or ISO along with 100 µM PAGln (Red), in β2-Tango transfected cells (P < 0.0001; B_max Blue vs Red). B β-arrestin2 recruitment induced by increasing concentration of isoproterenol (ISO) alone (Blue) or ISO along with 100 µM PAGln (Red), in β1-Tango transfected cells (P = 0.7; B_max Blue vs Red). C Norepinephrine (NE) alone (Blue) and NE along with 100 µM PAGln (Orange) induced dose-response of β-arrestin2 recruitment in β2-Tango transfected cells (P < 0.0001; B_max Blue vs Red). D Norepinephrine (NE) alone (Blue) and NE along with 100 µM PAGln (Red) induced dose-response of β-arrestin2 recruitment in β1-Tango transfected cells (P = 0.3; B_max Blue vs Red). β-arrestin2 recruitment was measured as relative luminance unit (RLU). Data points represent the mean ± SD of the indicated number (n) of experiments. P values (top left) for B_max comparisons (Blue vs Red) as well as the EC₅₀s and the standard error of the mean EC₅₀ (SEM) were determined with a non-linear regression method. Nonparametric two-tailed Mann–Whitney test was used for non-pairwise comparisons (*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001). All reported P values are two-sided. A P-value of <0.05 was considered significant in this study.