Fig. 5: Effect of PAGln and PAGly on ISO-induced mouse cardiomyocyte contractility.

A Experimental design of mouse ventricular cardiomyocyte contractility assay. Ventricular cardiomyocytes were isolated from adult C57BL/6 mice and contractility was measured using an IonOptix System under various conditions as indicated. B Representative cardiomyocyte contractility trace displaying changes in sarcomere length over time. C Cardiomyocyte contractility trace of ventricular cardiomyocytes from WT C57BL/6 mice after treating them with 10 µM ISO (Blue), 10 µM ISO with 100 µM PAGln (Red), 100 µM PAGln alone (Purple), or vehicle control (Black). D Quantification of the sarcomere shortening of each cardiomyocytes form (C) expressed as bar graph. E Contractility trace of ventricular cardiomyocyte from WT C57BL/6 mice after treatment with 10 µM ISO (Blue), 10 µM ISO with 100 µM PAGly (Red), 100 µM PAGly alone (Purple), and vehicle control (Black). F Quantification of sarcomere shortening of each cardiomyocytes form (E) expressed as bar graph. Data points represent the mean ± SD (n = cardiomyocytes from at least 3 mice). The nonparametric two-tailed Mann–Whitney test was used for non-pairwise comparisons and the two-sided Kruskal–Wallis test with Dunn’s post hoc test was performed for multiple comparisons in (D) (P < 0.0001) and (F) (P = 0.0008). All reported P values are two-sided. A P-value of <0.05 was considered significant in this study (*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001).