Fig. 1: Epithelial heterozygous deletion of Kmt2d promotes mouse HNSCC invasive growth and lymph node metastasis. | Nature Communications

Fig. 1: Epithelial heterozygous deletion of Kmt2d promotes mouse HNSCC invasive growth and lymph node metastasis.

From: Histone-methyltransferase KMT2D deficiency impairs the Fanconi anemia/BRCA pathway upon glycolytic inhibition in squamous cell carcinoma

Fig. 1

a Experimental design for 4NQO-induced primary mouse HNSCC. b Representative image of tongue lesions and quantification of lesion areas. Scale bar, 2 mm. Values are mean ± SD. n = 11:10. **p < 0.01 by unpaired two-tailed Student’s t test. c Representative IHC staining images of KMT2D in mouse HNSCC were selected from two independent experiments. n = 11:10. Enlarged images are in inserts. Scale bar, 50 µm. d Representative H&E staining of HNSCC and quantification of HNSCC number and area. Scale bar, 200 µm. Enlarged images are in the right panel. Scale bar, 50 µm. n = 11:10. **p < 0.01 by unpaired two-tailed Student’s t test. e Quantification of HNSCC invasion grades. *p < 0.05 by two-sided Cochran-Armitage test. f Representative IF staining and quantification of Ki-67 positive cells. Scale bar, 50 µm. Values are mean ± SD. n = 8:8. **p < 0.01 by unpaired two-tailed Student’s t test. g IHC staining of PCK positive cells and quantification of the metastatic area in cervical lymph nodes. Scale bar, 200 µm. Values are mean ± SD. n = 11:10. **p < 0.01 by unpaired two-tailed Student’s t test. h Quantification of metastatic lymph nodes. **p < 0.01 by two-sided chi-square test. i Experimental design for studying the effects of Kmt2d on the growth of 4NQO-induced mouse HNSCC. j Representative image of tongue lesions and quantification of lesion areas. Scale bar, 2 mm. Values are mean ± SD. n = 12:12. **p < 0.01 by unpaired two-tailed Student’s t test. k Representative H&E staining of HNSCC and quantification of HNSCC number and area. Scale bar, 200 µm. Enlarged images are in the right panels. Scale bar, 50 µm. Values are mean ± SD. n = 12:12. *p < 0.05, **p < 0.01 by unpaired two-tailed Student’s t test. l Quantification of HNSCC invasion grades. *p < 0.05 by two-sided Cochran-Armitage test. m IHC staining of PCK positive cells and quantification of metastatic area in lymph nodes. Scale bar, 200 µm. n = 12:12. **p < 0.01 by unpaired two-tailed Student’s t test. n Quantification of metastatic lymph nodes. *p < 0.05 by two-sided chi-square test. Source data are provided as a Source Data file.

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