Fig. 3: mmpReg and blastema formation.

a KEGG pathway categories of frequently detected genes differentially expressed between regenerating and intact animals. b The KEGG pathway subcategories of frequently detected genes are categorized as environmental information processing in the upper panel. c Schematic of the predicted MmpReg structure of E. japonensis. d Expression level of mmpReg during regeneration. mmpReg was upregulated after 2, 6, and 24 hpa. ***p < 0.001 (Dunnett’s test). Error bars indicate S.E.M. n = 5 for intact animals and for other time points. e, f mmpReg expression during blastema formation at 1, 5, 8, and 24 hpa using horizontal sections (e) and sagittal sections (f). Dashed lines indicate amputation sites. Scale bars represent 100 µm (e, f). A: anterior, P: posterior, D: dorsal, V: ventral (e, f). Source data are provided as a Source Data file.