Fig. 5: Distribution of proliferating cells in blastema formation. | Nature Communications

Fig. 5: Distribution of proliferating cells in blastema formation.

From: SoxC and MmpReg promote blastema formation in whole-body regeneration of fragmenting potworms Enchytraeus japonensis

Fig. 5

a Schematic drawing of EdU labelling. Small arrows indicate the fixation points with PFA–PBS. b, c, d Representative images of regenerating animals at 3, 5, 8 and 24 hpa. Bright-field images (b). The nuclei were stained with DAPI (c). EdU-incorporated cells (d). e, f, g, h The number of cells increased in both anterior blastema (e, 3 hpa (n = 18), 5 hpa (n = 32), 8 hpa (n = 18), 24 hpa (n = 25)) and posterior blastema (g, 3 hpa (n = 19), 5 hpa (n = 32), 8 hpa (n = 18), 24 hpa (n = 25)). The number of proliferating cells did not significantly increase (0–3 vs 3–5, 0–3 vs 5–8 hpa), but increased at 21–24 hpa (0–3 vs 21–24 hpa) in anterior and posterior blastema (d, f, h). *p < 0.05, **p < 0.01, ***p < 0.001. NS indicates not significant (Dunnett’s test). Representative of two independent experiments (e, f, g, h). Error bars: mean ± SD (e, f, g, h). Scale bars represent 250 µm (c, g, j, l, n). Dashed lines indicate amputation sites (b, c, d). Dotted lines indicate the outline of regenerating worms (c, d). Ventral views (b, c, d). Source data are provided as a Source Data file.

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