Fig. 7: Expression of GMP genes during blastema formation in E. japonensis.

a–e GMP gene (Ej-vasa1 (a), Ej-nanos (b), Ej-pl10 (c), Ej-piwi (d), Ej-vasa2 (e)) expression during blastema formation at 0, 1, 5, and 24 hpa in horizontal sections (upper eight panels) and sagittal sections (lower eight panels). Dashed lines indicate amputation sites. f GMP gene expression in gonad and PGZ at 0 hpa. Expression level varies depending on the GMP gene, but all GMP genes were expressed in the gonad and PGZ, except for Ej-piwi in PGZ at 0 hpa. Arrowheads in the left panels indicate the position of the gonad, and arrowheads in the right panels indicate the position of PGZ. g GMP gene expression in neoblast at 0 hpa. Ej-vasa1 was expressed in neoblasts, whereas the other GMP genes were not detected in neoblasts at 0 hpa. The regions around the septa are shown in each panel. Dotted lines indicate the outline of neoblasts. Scale bars represent 250 µm (a–f) and 20 µm (g). A: anterior, P: posterior, D: dorsal, V: ventral (a–f). All images are representative of at least two independent experiments (a–g).