Fig. 6: Phospho-S164 enhances T170 phosphorylation of Cdk7 in human cells.
From: Structural basis of Cdk7 activation by dual T-loop phosphorylation
a Phos-tag-SDS-PAGE and conventional SDS-PAGE immunoblot analysis of HCT116 whole-cell lysate. Membranes were probed with α-Cdk7 antibody to visualize total Cdk7 and in parallel with phospho-specific antibodies recognizing Cdk7 pT170 or Cdk7 pS164. Unspecific bands are marked by asterisks. b Conventional immunoblot and phos-tag immunoblot analysis of HCT116 cell lysates transfected with Cdk7-HA T-loop mutants. Blots were probed with α-HA antibody to visualize transfected Cdk7-HA and in parallel with phospho-specific antibodies recognizing Cdk7 pT170 or Cdk7 pS164. c Theoretical Cdk7 T loop phospho-isoforms, indicating possible pathways of sequential phosphorylation of S164 and T170.
