Fig. 2: Fewer CD8⁺ T cells, and decreased CD4⁺CD25⁺ and CD8⁺CD122^hi T cell numbers in Stat5a KI and Stat5b KI mice.

a Representative flow cytometric profiles of splenic CD4⁺ and CD8⁺ T cells in WT and Stat5a KI (top panels) and WT and Stat5b KI (bottom panels) mice. b Summary of the frequencies (left graph) and numbers (right graph) of splenic CD4⁺ and CD8⁺ T cells in WT and Stat5a KI and Stat5b KI mice (n = 13 for Stat5a WT CD4⁺ T cells, n = 12 for Stat5a KI CD4⁺ T cells, n = 12 for Stat5b WT CD4⁺ T cells, n = 12 for Stat5b KI CD4⁺ T cells, n = 13 for Stat5a WT CD8⁺ T cells, n = 12 for Stat5a KI CD8⁺ T cells, n = 11 for Stat5b WT CD8⁺ T cells, and n = 12 for Stat5b KI CD8⁺ T cells). c Representative flow cytometric profiles of splenic CD4⁺CD25⁺ T cells in WT and Stat5a KI (left panels) and WT and Stat5b KI (right panels) mice. d Summary of the frequencies (left graph) and numbers (right graph) of spleen CD4⁺CD25⁺ T cells in WT and Stat5a KI and Stat5b KI mice (n = 12 for both Stat5a WT and Stat5a KI mice and n = 6 for both Stat5b WT and Stat5b KI mice). e Representative flow cytometric profiles of spleen CD4⁺Foxp3⁺ T cells in WT and Stat5a KI (left panels) and WT and Stat5b KI (right panels) mice. f Summary of the frequencies (left graph) and numbers (right graph) of spleen CD4⁺Foxp3⁺ T cells in WT and Stat5a KI and Stat5b KI mice (n = 12 for Stat5a WT mice, n = 11 for Stat5a KI mice, n = 8 for both Stat5b WT and Stat5b KI mice). g Representative flow cytometric profiles of spleen CD8⁺CD122^hi T cells in WT and Stat5a KI (left panels) and WT and Stat5b KI (right panels) mice. h Summary of the frequencies (left graph) and numbers (right graph) of spleen CD8⁺CD122^hi T cells in WT and Stat5a KI and Stat5b KI mice (n = 13 for Stat5a WT, n = 11 for Stat5a KI, n = 11 for Stat5b WT, and n = 12 for Stat5b KI). i Representative flow cytometric profiles of T (left four panels) and CD8⁺ T cells (right four panels) in WT and Stat5a KI (upper panels) and Stat5b KI (lower panels) mice on day 7 after PBS or IL-2 injection. j Bar graphs showing numbers of CD3⁺ and CD3⁺CD8⁺ T cells in WT and Stat5a KI and Stat5b KI mice on day 7 after PBS (n = 3) or IL-2 injection (n = 5). The gating strategies for a, c, e, g, and i are shown in Supplementary Fig. 8. The numbers in a, c, e, g, and i are percentage of gated cells; in graphs b, d, f, h, and j, black and red open bars represent WT and mutants, respectively, and the error bars (SD) and p-values are shown, which were determined by multiple unpaired t test using two-stage step-up method of Benjamini, Krieger and Yekutieli. Source data for b, d, f, h, and j are provided as a Source Data File.