Fig. 1: Anatomical distribution of PL projections to the PVT and avTRN.
a Schematic of the viral vector strategy used for anterograde tracing of PLL5 (Rbp4-Cre) or PLL6 (Syt6-Cre) projections to the PVT and avTRN. b Representative images showing mCherry expression in Layer 6 neurons of the PL (left) and axon terminals within the PVT (middle) and the avTRN (right). c Representative images showing mCherry expression in Layer 5 neurons of the PL (left) and axon terminals within the PVT (middle) and the avTRN (right). d Schematic of the retrograde tracing strategy used for labeling avTRN- and PVT-projecting PL neurons. e Representative images showing the targets for dual-color CTB injections, green into the avTRN (left) and red into the PVT (right). f, Representative images showing the retrograde labeled avTRN-projecting cells (green) and PVT-projecting cells (red) are present in the PL region. g Left: Quantification of the density of avTRN- and PVT-projecting neurons in the PL (n = 3 mice, 4 slices per subject) one-way ANOVA, F(2, 33) = 28.15, P < 0.0001. Right: Quantification of the percentage of double-projecting cells in the PL. two-tailed t test, t(22) = 4.55, **P = 0.0046. h Quantification of PL cell counts for each projector, normalized by projector type, plotted along the distance from midline. n = 3 mice. i Schematic of the viral vector strategy to trace the PL inputs to PVT–projectors in the avTRN. j Representative image showing the rabies starter cells (Rabies-GFP and TVA-mCherry double-labeled cells) in avTRN region. k Representative images showing the projectors (Rabies-GFP) of avTRN PVT-projecting cells in the PL. All anatomical experimented were repeated at least once, and similar results were obtained. Data are shown as mean ± s.e.m. Source data are provided as a Source Data file.
