Fig. 3: Optogenetic stimulation of the PL drives inhibitory responses in the PVT that are partially rescued by chemogenetic inhibition of avTRN. | Nature Communications

Fig. 3: Optogenetic stimulation of the PL drives inhibitory responses in the PVT that are partially rescued by chemogenetic inhibition of avTRN.

From: Convergent direct and indirect cortical streams shape avoidance decisions in mice via the midline thalamus

Fig. 3

a Schematic of the experimental approach for fiber photometry imaging of NAc-projecting PVT cells in response to PL stimulation with and without avTRN inhibition. b Representative image of ChR2-YFP expression and optical fiber placement in the PL region (top), ChR2-YFP (green), mCherry-hM4Di (red), and anti-PV (white) expression in TRN region (middle), and GCaMP8s, ChR2-YFP (green), and mCherry-hM4Di (red) expression and fiber placement in pPVT (bottom). c–e Calcium signal quantification during PL opto-activation and avTRN chemogenetic-inhibition. PL with ChR2-YFP and TRN with mCherry-hM4Di (Gi), n = 4 mice (c), PL with ChR2-YFP and TRN with mCherry, n = 4 mice (d) and PL with YFP and TRN with Gi, n = 3 mice. e Left: Heatmaps of individual trial calcium responses for Saline and CNO treatment. Middle: Average calcium signal and optogenetic (opto) stimulation duration (blue background). Right: Calcium signal AUC quantification during pre-opto, opto and post-opto, Mixed-effects model (REML) n = 60 Trials from 4 mice, F(2, 236) = 10.65, P < 0.0001. d: n = 60 Trials from 4 mice, F(2, 236) = 0.98, P = 0.38. e: n = 45 Trials from 3 mice, F(2, 176) = 1.87, P = 0.16. For all quantifications, multiple comparisons were conducted corrected by two-stage linear step-up procedure. Asterisks indicate where ANOVA multiple comparisons found significance. Data are shown as mean ± s.e.m. Source data are provided as a Source Data file.

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