Fig. 5: Selection of improved NS from FgAA-containing nanobody libraries.
From: Rapid discovery and evolution of nanosensors containing fluorogenic amino acids
a The experimental strategy to evolve nanobodies containing nsAAs by mRNA/cDNA display. Panel created with help from BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en). b NS sequences were enriched when selecting for the RBDOB.1 antigen. c Dose response curves with evolved NS (Omicron-NS-1, 2, and 3) showed high affinity and fluorescence fold improvements relative to CoV19-NS when exposed to RBDOB.1. Each of the relative fluorescence units in the graph were normalized relative to the maximum response of Omicron-NS-2 for comparisons of relative NS brightness. Lines represent a 4PL fit of the dose-response curves. Shaded areas and dashed lines represent the 95% confidence intervals of the fits. Bars and error bars represent the average and standard deviation of independent triplicate experiments. Source data are provided as a Source Data file.
