Fig. 6: Immunogenicity of HexaPro-SS and HexaPro-SS-2W constructs.
From: Simulation-driven design of stabilized SARS-CoV-2 spike S2 immunogens
a, b Sera from mice immunized with HexaPro-SS-2W can neutralize rVSV-CoV-2 variants. a 6–8-week-old female C57BL/6J mice (n = 6) were primed (week 0) and boosted (week 3) with 10 µg of immunogens HexaPro-SS, HexaPro-SS-2W, or PBS. b Sera was isolated at week 7 and evaluated for neutralization capacity against rVSVs bearing the spike proteins of SARS-CoV-2 Wuhan-1 or SARS-CoV-2 Omicron BA.1. Lower area under the curve (AUC) values correspond to better sera neutralizing capability. AUCs were compared across groups by ordinary one-way ANOVA with Tukey’s multiple comparisons test (** indicates a p-value < 0.01; n.s. indicates not significant). c Biolayer interferometry sensorgrams showing binding responses (colored lines indicate 1:1 binding fit, black lines indicate reference subtracted response) of previously reported neutralizing or non-neutralizing antibodies to epitopes on the S2 subunit in HexaPro-SS (top) and HexaPro-SS-2W (bottom) constructs.
