Fig. 3: HDSP exerts oncogenic effects in gastric cancer.

a, b The HDSP or HOXA10-HOXA9 mut expression plasmids was reintroduced into GC cells with stable low HDSP levels. Thus, the GC cells were divided into four groups: Ctrl, HOXA10-HOX9KD, KD + HDSP, and KD + HOXA10-HOXA9 mut. Proliferative activity of above four groups of GC cells were evaluated using CCK-8 (a) and colony formation (b) assays. Mean ± SD, two-tailed Student’s t test, n = 3 biologically independent experiments. ns, not significant; *P < 0.05, **P < 0.01. c Subcutaneous injection of above four groups GC of cells into nude mice was performed and representative images of tumors in xenograft model were shown. d Absolute tumor volume presented as a mean ± SD, n  =  4 biologically independent animals. ns, not significant; **P < 0.01. e Tumor weight was measured after 29 days. Mean ± SD, two-tailed Student’s t test, n  =  4. ns, not significant; *P < 0.05. f Representative H&E staining of xenograft tissues and IHC staining of Ki67 are shown. Scale bar: 50 μm. g, h Transwell assays were conducted to analyze the migration and invasion ability of above four groups of GC cells. Scale bar: 100 μm. Mean ± SD, two-tailed Student’s t test. n = 3 biologically independent experiments. ns, not significant; *P < 0.05. i BLI showing lung metastasis in vivo. Mean ± SD, two-tailed Student’s t test. n = 3 biologically independent animals. ns, not significant; *P < 0.05. j H&E staining to detect the number and size of nodule production in the lung metastasis model. Scale bar: 500 μm. NC negative control, KD knock down. Source data are provided as a Source data file.