Fig. 5: CITE-seq analysis of memory B cells and plasmablasts in COVID-19, Sepsis, and vaccination.

A Schematic of CITE-seq experiment. FACS sorting strategy in Supplementary Fig. 6. B Weighted nearest neighbor (wnn) UMAP of IgD- memory B cells in CITE-seq colored according to SLM clusters. Dot plot of expression of selected differentially expressed mRNA. C Single cell Violin plots of Antibody Derived Tag (adt) protein markers. Selected other mRNA and protein markers shown in Supplementary Fig. 7A and (E). D Expression of indicated markers displayed on wnnUMAP. Other markers are shown in Supplementary Fig. 7B. E Volcano plots of differentially expressed genes between indicated populations. Other comparisons shown in Supplementary Fig. 7D. Figure 5A Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en).