Fig. 8: Analysis of tetramer reactive B cells by CyTOF confirms that CD45RBlo groups are the main SARS-CoV2 reactive memory B cells pre and post tertiary vaccination. | Nature Communications

Fig. 8: Analysis of tetramer reactive B cells by CyTOF confirms that CD45RBlo groups are the main SARS-CoV2 reactive memory B cells pre and post tertiary vaccination.

From: Atypical and non-classical CD45RBlo memory B cells are the majority of circulating SARS-CoV-2 specific B cells following mRNA vaccination or COVID-19

Fig. 8

A Gating of SARS-CoV2 tetramer-bound B cells. Cells past the indicated thresholds were considered positive. B Percent wtSpike positive cells per total B cell of the indicated cell type (Naive, Memory or Plasmablasts) per total B cells. (n = 14). C Percent wtSpike positive, wtRBD positive and B1.1.529-RBD positive cells of the indicated cell type per total (B) memory cells (excluding Naive and Plasmablasts). (n = 14). D Median proportions of (B) memory clusters of wtSpike negative and indicated tetramer positive cells pre and post third vaccination (n = 14). E Median proportions of B memory clusters of wtSpike negative and indicated tetramer positive cells for COVID-19 (n = 3) Significance *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 by two-sided Paired Wilcoxon (B, C, D). Box plots are median +/-  interquartile range (IQR), whiskers are 1.5 * IQR.

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