Fig. 4: Computational pathology modeling recapitulates the sexual dimorphism of TNF-Tg inflammatory arthritis.

An independent set of slides from the training slides were used to validate the cell type prediction model (3 months-old: WT Male n = 5, WT Female n = 4, TNF-Tg Male n = 6, TNF-Tg Female n = 5; 4 months-old: WT Male n = 6, WT Female n = 4, TNF-Tg Male n = 5, TNF-Tg Female n = 5; 5.5 months-old: WT Male n = 4, WT Female n = 5, TNF-Tg Male n = 6, TNF-Tg Female n = 6). Tissue segmentation was first performed to segment the synovium and then cell type predictions were performed within the synovium. A Lymphocytes predictions counts. Each dot is one mouse, Box and Whisker plots are construct by showing the Min, 25th percentile, Median, 75th percentile and Max. Please note the log scale. Lymphocyte counts were found to be lognormal, a log transformation was performed on the data and a Two-Way ANOVA with Tukey’s Post-hoc test was conducted. B Synovial Lining Cell prediction counts. Each dot is one mouse, Box and Whisker plots are construct by showing the Min, 25th percentile, Median, 75th percentile and Max. Please note the log scale. A Two-Way ANOVA with Tukey’s Post-hoc test was conducted. Differences only shown between the female and male TNF-Tg mice. C Fibroblast predictions counts. Each dot is one mouse, Box and Whisker plots are construct by showing the Min, 25th percentile, Median, 75th percentile and Max. A Two-Way ANOVA with Tukey’s Post-hoc test was conducted. Differences only shown between the female and male TNF-Tg mice. D Lymphocyte predictions compare to the synovial inflammatory score as previously quantified in Figure 2F of Bell et al.¹⁹. Lymphocyte counts were not normally distributed. Spearman’s correlation, TNF-Tg mice only (n = 28). Please note the log scale on the x-axis. E Synovial Lining cell counts compared to the pannus invasion score as previously quantified in Supplemental Figure 3I in Bell et al.¹⁹. Synovial Lining cell counts were not normally distributed. Spearman’s correlation, TNF-Tg mice only (n = 28). Please note the log scale on the x-axis. F Total cell counts in the synovium compared to the cell area as previously quantified in Figure 2D of Bell et al.¹⁹. Total cell counts and cell area in the synovium were normally distributed. Pearson’s correlation, TNF-Tg mice only (n = 28).