Fig. 1: HiUGE-iBioID reveals endogenous proximity proteomes of 14 autism risk proteins. | Nature Communications

Fig. 1: HiUGE-iBioID reveals endogenous proximity proteomes of 14 autism risk proteins.

From: Proximity analysis of native proteomes reveals phenotypic modifiers in a mouse model of autism and related neurodevelopmental conditions

Fig. 1

A Schematic illustration of HiUGE-iBioID and its workflow. GS-sgRNA: gene-specific-gRNA. DS-sgRNA: donor-specific-gRNA. Strategies to preserve C-term PDZ-binding motifs of Syngap1, Ctnnb1, Iqsec2, and Lrrc4c are detailed. B Overview of 14 proximity proteomes that segregates according to expected bait functions. C Enrichment analysis of overlapping SFARI genes using hypergeometric probability. The solid red line denotes Bonferroni adjusted p-value at 0.05. D Proximity proteome clustering based on a similarity matrix. E Core proximity proteome between Syngap1 and Anks1b that show highly significant overlaps with SFARI genes. F Core proximity proteome amongst Ank3, Scn2a, and Scn8a that are clustered based on similarity. Modules of proteins were isolated by MCL clustering or GO analysis.

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