Fig. 4: Mechanism of two-level compartmentalization.
From: Thermo-responsive aqueous two-phase system for two-level compartmentalization

a Schematic of two-level compartmentalization. b ATR-FTIR spectra of the PNIPAM (5 wt%)/DEX (5 wt%) aqueous solution at 25 °C and 35 °C. c 1H-NMR spectroscopy of the 5 wt% PNIPAM, 5 wt% DEX, and PNIPAM (5 wt%)/DEX (5 wt%) aqueous solution at 25 and 35 °C. d Phase-separated PNIPAM droplets in PNIPAM (5 wt%)/PAM (5 wt%) solution under FITC channel, rhodamine channel, and merged channel at 25 and 35 °C. Each experiment was repeated independently three times with similar results. e Phase-separated PEG droplets in PEG (1 wt%)/DEX (9 wt%) solution under FITC channel, rhodamine channel, and merged channel at 25 and 35 °C. Each experiment was repeated independently three times with similar results.