Fig. 1: Morphometric analysis of the SAM reveals volumetric shrinkage at the deep boundary.
From: Water fluxes pattern growth and identity in shoot meristems
A Processed signal of pUBQ10::LTi6B-TdTomato marker used for 3D segmentation (L1 only shown). B Heatmaps showing cell volumes from the top view (L1 only) and from an orthogonal view obtained at the site of the black arrows. C Heatmaps showing the surface mean curvature and the organ stages 1 (S1) and 2 (S2). D Heatmap showing cell sphericity. E Heatmap showing the sphericity changes over 12 h. F Heatmap showing volumetric growth rates over 12 h. Dividing cells appear in white, so growth rates are only shown for non-dividing cells. G Growth rate maps of a representative organ-meristem region including dividing cells (same sample as in panel F). The volume of daughter cells was added to obtain tissue-level growth rate. Note in panel (F) that no cell division was detected in the boundary. The subset of cells with negative curvature (left) and positive curvature (right). H Boxplot shows the position-dependent differential growth rates of cells for one meristem. The box indicates the interquartile range (IQR), the whiskers show the range of values within 1.5*IQR, and a horizontal line indicates the median. A similar heatmap profile was detected in all studied meristems (n = 6). I Scatter plot showing growth rate vs. sphericity change for cells quantified in (H).
