Fig. 4: eBICS is resorbed after 7 days and can be applied to larger animals. | Nature Communications

Fig. 4: eBICS is resorbed after 7 days and can be applied to larger animals.

From: In situ assembly of an injectable cardiac stimulator

Fig. 4

a, b Dissected hearts of BICS-implanted zebrafish at (a) 1 h post-injection (hpi) (B: bulbus arteriosus, V: ventricle) and (b) 7 dpi. Complete resorption at 7 dpi is evident from the lack of black BICS hydrogel. (c–f) Immunohistofluorescence (anti-myeloperoxidase) images of zebrafish hearts at 1 hpi, 1 dpi, 2 dpi, and 7 dpi, respectively (n ≥ 3 for each time point). Scale bars denote 100 µm. g Cell viability of HFL-1 fibroblast cells after treatment with the indicated concentrations of ETE-BuSA or proBICS for 24 h. For proBICS, the concentrations are presented as [ETE-BuSA]/[A5]. (n = 3 independent biological replicates each with 3 technical replicates). Untreated cells were used as control. Data are presented as mean values ± SEM.” h, i Comparison of the anatomy of (h) the chicken heart and (i) a human heart model. The compartments are labeled (RA: right atrium, RV: right ventricle, LA: left atrium, LV: left ventricle). j Chicken heart with BICS placed at the atrium. k BICS injected chicken embryo, eBICS patch on the skin as and the formation of eBICS around the heart presented (zoomed in picture).

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