Fig. 5: Chloroplastic 1O2 burst upon NAD+ depletion activates jasmonate biosynthesis.
From: NAD+ deficiency primes defense metabolism via 1O2-escalated jasmonate biosynthesis in plants
Contents of α-LeA (a), 13-HPOT (b), 12-OPDA (c), JA (d) and JA-Ile (e) in the leaves of Col-0, qs-2, rbohF3 and qs-2rbohF3 plants. The data represent the means ± SD (n = 6 biological replicates), two-sided Student’s t test. f A heatmap showing the relative contents of four key GSLs in the rosette leaves of Col-0, qs-2, rbohF3 and qs-2rbohF3 plants. g Analysis of maximum photochemical efficiency of PSII (Fv/Fm) in qs-2 mutant, COM1 and Col-0 wild-type plants. The qs-2, COM1 and Col-0 plants were grown in soil under normal growth conditions for 21 days, then the mature rosette leaves were analyzed by a portable fluorometer (JUNIOR-PAM, Walz, Germany). n = 9, two-sided Student’s t test. h Representative confocal images showing SOSG fluorescence in leaf mesophyll cells of 3-week-old Col-0, qs-2, COM1, COM2, rbohF3 and qs-2rbohF3 plants. Scale bars, 20 μm. i Summary of the accumulation of different ROSs in the rosette leaves of indicated genotypes. Contents of α-LeA (j), 13-HPOT (k), 12-OPDA (l), JA (m) and JA-Ile (n) in the leaves of Col-0 and lox2-c1 with DCMU treatment for indicated hours. The data represent the means ± SD (n = 4), and the significance analysis was performed by two-tailed Student’s t test. Source data are provided as a Source Data file.
